AccuPrime™ Taq DNA Polymerase High Fidelity amplifies nucleic acid templates using antibody-mediated hot-start, a blend of Taq DNA Polymerase and proofreading enzyme, and AccuPrime™ accessory proteins for improved PCR fidelity, yield, and specificity over other hot-start DNA polymerases. With AccuPrime™ Taq DNA Polymerase High Fidelity you'll get:
The highest specificity and yield for the most robust PCR amplification
9-fold higher fidelity than Taq DNA polymerase alone
Mimimal optimization steps, even with non-optimized primer sets
Efficient amplification of targets over a broad size range up to 20 kb
High fidelity is achieved by a combination of Platinum® anti-Taq DNA polymerase antibodies that inhibit polymerase activity, providing an automatic "hot-start", and the proofreading (3´a5´ exonuclease activity) enzyme Pyrococcus species GB-D. The thermostable AccuPrime™ accessory proteins enhance specific primer-template hybridization during every cycle of PCR, preventing mispriming and enhancing PCR specificity and yield (Figure 1).
Contents and Storage
AccuPrime™ Taq DNA Polymerase High Fidelity is supplied with MgSO4 and two 10X reaction buffers containing dNTPs. Buffer I is optimized for plasmids, cDNA, and λ DNA. Buffer II is optimized for genomic DNA.
Store at -20° C. Guaranteed stable for 6 months when properly stored.
原厂资料：
AccuPrime™ Taq DNA Polymerase High Fidelity amplifies nucleic acid templates using antibody-mediated hot-start, a blend of Taq DNA Polymerase and proofreading enzyme, and AccuPrime™ accessory proteins for improved PCR fidelity, yield, and specificity over other hot-start DNA polymerases. With AccuPrime™ Taq DNA Polymerase High Fidelity you'll get:
The highest specificity and yield for the most robust PCR amplification
9-fold higher fidelity than Taq DNA polymerase alone
Mimimal optimization steps, even with non-optimized primer sets
Efficient amplification of targets over a broad size range up to 20 kb
High fidelity is achieved by a combination of Platinum® anti-Taq DNA polymerase antibodies that inhibit polymerase activity, providing an automatic "hot-start", and the proofreading (3´a5´ exonuclease activity) enzyme Pyrococcus species GB-D. The thermostable AccuPrime™ accessory proteins enhance specific primer-template hybridization during every cycle of PCR, preventing mispriming and enhancing PCR specificity and yield (Figure 1).
Contents and Storage
AccuPrime™ Taq DNA Polymerase High Fidelity is supplied with MgSO4 and two 10X reaction buffers containing dNTPs. Buffer I is optimized for plasmids, cDNA, and λ DNA. Buffer II is optimized for genomic DNA.
Store at -20° C. Guaranteed stable for 6 months when properly stored.
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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