Iscove’s Modified Dulbecco’s Medium (IMDM) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. Life Technologies offers a variety of Gibco® IMDM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This IMDM is modified as follows:
With
Without
• L-glutamine
• α-thioglycerol
• Phenol Red
• 2-mercaptoethanol
The complete formulation is available.
IMDM, a modification of Dulbecco’s Modified Eagle Medium, includes selenium as well as additional amino acids and vitamins. In addition, this unique medium lacks iron, with potassium nitrate replacing ferric nitrate.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of View Morelocal law.
cGMP Manufacturing and Quality System
Gibco® IMDM is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers an identical Gibco® IMDM product made in our Scotland facility (42200-030). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
IMDM contains no proteins, lipids, or growth factors. Therefore, IMDM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). IMDM uses a sodium bicarbonate buffer system (3.024 g ⁄ L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
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