ELECTROMAX DH10B T1 CELLS 0.5 ML (5 X 0.1 ML)

• 产品编号：12033015      品牌：invitrogen       原厂货号：12033015
• 产品分类：克隆 > 克隆载体和克隆试剂盒 > 细菌菌株和感受态细胞 > 感受态细胞
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描述：

DH10B™ T1 Phage-Resistant E. coli have all the benefits of the DH10B™ genotype, with the addition of the tonA genotype which confers resistance to T1 and T5 phage infection. The inclusion of the tonA genotype protects your valuable samples and minimizes the possibility of downtime in your lab due to phage contamination. The DH10B™-T1^R genotype has the following features:

 

• tonA genotype to confer resistance to T1 and T5 phage

• Elimination of mcrA, mcrBC, mrr, and hsdRMS restriction systems to allow construction of more representative genomic libraries (1,2)

• The endA1 mutation to increase plasmid yield and quantity

ZΔM15 for blue/white screening of recombinant clonesDH10B™-T1^R E. coli are available in both electrocompetent and chemically competent formats. The high transformation efficiency makes them ideal for generating cDNA or genomic libraries. High efficiency transformation is seen with plasmids View More150 kb in size (3).

原厂资料：

描述

ElectroMAX™ DH10B™ T1 Phage-Resistant electrocompetent E. coli cells offer transformation efficiencies that are among the highest achieved by any product offered by Life Technologies, and can exceed 1 x 10¹⁰ cfu/µg plasmid DNA in efficiency. They are ideal for applications requiring high efficiency transformation, including cDNA and gDNA library construction.

• Maximizes yield of transformants from limited cloning products
• Permits efficient cloning of methylated DNA
• Resistant to T1 and T5 bacteriophages
• Supports blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal
• Delivers high yield plasmid preparations for downstream applications

Phage-Resistant Representative Libraries from a Single Transformation
The tonA mutation in DH10B™ confers resistance to bacteriophages T1 and T5 (1), safeguarding valuable libraries against contamination. Additionally, mutations in the methylation-dependent restriction system (mcrA, mcrBC, and mrr) make ElectroMAX™ DH10B™ T1 Phage-Resistant Cells ideal for construction of genomic libraries of both prokaryotic and eukaryotic genomic DNA, and allow for efficient plasmid rescue from eukaryotic genomes (2). ElectroMAX™ DH10B™ T1 Phage-Resistant Cells are suitable for construction of gene banks, for the generation of cDNA libraries using plasmid-derived vectors, and for applications where there DNA is limiting. The strain also has the Φ80lacZΔM15 genotype, providing for the option of blue/white screening on plates containing either X-Gal or Bluo-Gal. Finally, the endA1 mutation makes DH10B™ an excellent vehicle for amplifying plasmid DNA for subsequent extraction and purification.

Genotype:
F^- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139Δ(ara, leu)7697galU galK λ^- rpsL nupG tonA

Find the Strain and Format That You Need
DH10B™ cells are available in both electrocompetent and chemically competent formats. OurElectroMAX DH10B™ Cells are also suitable for cDNA or gDNA library construction but are not resistant to T1 and T5 phage infection. Our MegaX DH10B™ T1^R Electrocomp™ Cells have the highest transformation efficiency (>3 x 10¹⁰ cfu/µg) and also offer the benefit of T1 and T5 phage resistance.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

注意事项：

(Do not store in liquid nitrogen)