DMEM 10L, BAG

产品编号：11965175 品牌：invitrogen 原厂货号：11965175
产品分类：细胞,细胞培养与转染试剂 > 基础培养基 > DMEM
应用分类：细胞培养产品与服务 > 哺乳动物细胞培养 > 细胞培养基 > DMEM

包装：

运保温度:

2-8°C

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描述：

DMEM是广泛使用的哺乳动物细胞培养基。原代成纤维细胞、神经细胞、胶质细胞、人脐静脉内皮细胞、平滑肌细胞都可使用DMEM进行培养。

DMEM 配方改良如下：

With	Without
• High Glucose	• Sodium Pyruvate
• L-glutamine	• HEPES
• Phenol Red

可查看完整的配方。

DMEM 氨基酸和维生素浓度是传统 Eagle's Minimal 查看更多Essential Medium（EMEM）的四倍。 DMEM 最初配制含较低浓度的葡萄糖(1 g⁄L)和丙酮酸钠, 目前使用的DMEM多为高糖、有/无丙酮酸钠。

DMEM不含蛋白质、脂类、生长因子等。因此使用时需添加补充添加剂，一般为10%FBS。DMEM使用碳酸氢钠缓冲体系(3.7 g / L)，因此在 5-10% CO₂ 环境下培养细胞。

培养基配方

11965 - DMEM, high glucose

原厂资料：

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using themedia selector tool.

This DMEM is modified as follows:

With	Without
• High Glucose	• Sodium Pyruvate
• L-glutamine	• HEPES
• Phenol Red

The complete formulation is available.

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.

Product Use
For human ex vivo tissue and cell culture processing applications. CAUTION: When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using Gibco® DMEM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference our Type II Drug Master File (DMF).

cGMP Manufacturing and Quality System
Gibco® DMEM is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers an identical Gibco® DMEM product made in our Scotland facility (41965-062). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.