Temin's Modified Eagle Medium (MEM), is a modified form of Minimum Essential Medium, one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Life Technologies offers a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This MEM is modified as follows:
With
Without
• L-glutamine
• HEPES
• Earle's salts
• Phenol Red
• Sodium Bicarbonate
The complete formulation is available.
MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). 查看更多Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. Temin’s Modification is a 2x MEM with 4x the vitamins and amino acids of the original MEM. This MEM formulation contains Earle’s salts for use in a CO2 incubator.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing
Gibco® MEM is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards.
MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (8.4 g ⁄ L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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