Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. Life Technologies offers a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hank's salts for use without CO2.
Product Intended Use For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System Gibco® Medium 199 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers an identical Gibco® Medium 199 product made in our Scotland facility (21180-021). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g / L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Concentrated forms of Gibco® Medium 199 require sodium bicarbonate addition, pH adjustment, and filtration at the time of preparation (see protocol for details).
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For Research Use Only. Not for use in diagnostic procedures.
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