EXPRESS One-Step SuperScript® qRT-PCR SuperMix Kits incorporate proven Invitrogen enzymes now specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for your probe-based qPCR experiments.
These kits provide:
• Fast-activating, antibody-mediated Platinum® Taq DNA Polymerase—complete, rapid activation provides the best sensitivity and reproducibility
• The One-Step SuperScript® III Reverse Transcriptase module—a unique formulation to ensure better yield and less PCR inhibition, specifically for one-step qRT-PCR
• Flexible formats with ROX premixed or separate—designed to work with default instrument protocols on a wide range of high-throughput fast instruments (e.g., AB 7500 Fast, AB 7900 HT Fast, AB StepOne, Roche LightCycler® 480, Corbett Rotor-Gene™)
• The only commercially available one-step qRT-PCR kits containing heat-labile UDG—significantly reduced carryover contamination risk with excellent activity from room temperature to approximately 40°C. Due to the thermostability of SuperScript® III, the cDNA synthesis step is run at 50°C, at which temperature the heat-labile UDG is totally and irreversibly inactivated
Easy to test and switch
EXPRESS kits work with manufacturer’s default protocols on fast instruments and come in a convenient and stable SuperMix format for ultimate convenience. The kits are offered with either premixed ROX or a separate tube of ROX to fit your instrument needs.
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For Research Use Only. Not for use in diagnostic procedures.