Platinum® Quantitative RT-PCR ThermoScript™ One-Step System (1) is ideal for real-time quantitation of RNA molecules from total or poly(A)+ RNA in a single step. ThermoScript™ RT, a cloned avian reverse transcriptase, has been engineered to have reduced RNase H activity and high thermal stability (up to 70°C) to copy RNA with secondary structure and improve specificity of priming. Specificity and amplification efficiency are further enhanced through the use of Platinum® Taq DNA polymerase (3). The system can be used to quantitate the amount of specific mRNA from small numbers of cells (Figure 1). The Platinum® Quantitative RT-PCR ThermoScript™ One-Step System:
Is optimized to provide maximal performance with fluorescent detection chemistries employed in real-time quantitative PCR
Provides reduced RNase H activity for greater first-strand cDNA yield
Takes place sequentially in a single tube, under conditions optimal for both ThermoScript™ RT and