FastDNA 土壤基因组DNA纯化试剂盒(2 mL)FastDNA Spin Kit For Soil

Isolation of DNA from cells present in soil or other environmental samples The FastDNA® SPIN Kit for Soil quickly and efficiently isolates PCR-ready genomic DNA directly from soil samples in less than 30 minutes. Designed for use with any FastPrep® Instrument, plant and animal tissues, bacteria, algae, fungi spores and other members of a soil population are easily lysed within 40 seconds. These benchtop devices use a unique, optimized motion to homogenize samples by simultaneous impaction with lysing matrix particles. Up to 300 mg of soil samples are placed into 2.0 ml tubes containing Lysing Matrix E, a mixture of ceramic and silica particles designed to efficiently lyse all soil organisms including historically difficult sources such as eubacterial spores and endospores, gram positive bacteria, yeast, algae, nematodes and fungi. Homogenization takes place in the presence of MT Buffer and Sodium Phosphate Buffer, reagents carefully developed to protect and solubilize nucleic acids and proteins upon cell lysis. These reagents work together to allow extraction of genomic DNA with minimal RNA contamination. Following lysis, samples are centrifuged to pellet soil, cell debris and lysing matrix. The released DNA is purified by a silica-based GENECLEAN® procedure using SPIN filters and removes humic acids/polyphenols that can inhibit PCR. Eluted DNA is ready for PCR, restriction digest, electrophoresis and any other desired application.

 

土壤基因组DNA提取试剂盒

FastDNA® Spin Kit for Soil
　　　　
　　
　　用于从土壤中的所有生物包括G+细菌、酵母菌、真菌、藻类、线虫类甚至真细菌的孢子、芽孢中提取基因组DNA。而且它还可以提取其他环境样品的DNA，如沉积物、排泄物、废水、雪水等。只需500mg的样品即可得到足够的DNA。实验原理为用特殊的裂解介质E处理样品，之后使用自旋过滤器基于硅吸附DNA原理的GENECLEAN®步骤进行纯化，得到的DNA可用于PCR、限制性酶切、电泳及其他下游实验。

产品特点：
　　 配合FastPrep®仪器裂解样品处理更快、重复性更好、产量更高；
　　 适用于从不同类型土壤的微生物和动植物组织中分离基因组DNA；
　　 对不同来源的样品，都可以在60min内裂解分离DNA，配合FastPrep®仪器只需30min；
　　 不含有毒有害的试剂成分。

 

产品应用：

从土壤或其它环境样品的生物体中分离DNA,所获得的DNA可用于PCR、电泳、酶切和其它实验。

 

产品组分:

裂解介质E，磷酸钠缓冲液，MT溶液，PPS溶液， SEWS-M溶液，DES溶液，BBS胶上样缓冲液，磷酸钠缓冲液，纯化吸附硅砂，吸附柱，收集管，自旋过滤装置

保存条件：

试剂盒置于室温（15-30℃）条件下保存。

实例：下图的基因组DNA使用FastDNA® Spin Kit for Soil提取获得。

　　土壤样品来自：番茄盆栽；淤泥；砂土；松树下；棕榈树下；绿色花园；马蹄莲盆栽；草坪；柑橘树和鳄梨树等
土壤不难提。关键有两点：
1、土壤的使用量。如非肥沃（微生物含量多）的土壤则需要多用些土壤样品。
2、裂解。第一步裂解很重要。众所周知，土壤里的微生物悬浮不出来，细胞裂解不了，你要的DNA从何而来？所以需要充分涡旋、以保裂解。