Isolation of genomic DNA from plants, animals, bacteria, yeast, algae, and fungi using a silica spin filter method The FastDNA™ SPIN Kit, an updated version of the well-known FastDNA™ Kit, is used with any FastPrep® Instrument to lyse and subsequently isolate DNA from up to 200 mg of almost any sample in less than 30 minutes. Lysing Matrix A and three different chaotropic buffers are provided for the optimal homogenization of a wide variety of sample types (plant and animal tissues, cultured cells, bacteria, yeast, fungi, insects, etc..). The released DNA is purified by a silica based GeneClean® SPIN procedure, with supplied filter buckets and catch tubes to simplify liquid handling, and is suitable for PCR analysis and other downstream applications.
Matrix A, garnet matrix and one 1/4 inch ceramic sphere
Molecule to be Enriched
DNA
Kit Components
Lysing Matrix A 100 2.0 ml tubes, 1/4 Ceramic Spheres 100 spheres, Binding Matrix 66 ml, Concentrated SEWS-M 12 ml, DES 25 ml, CLS-VF 90 ml, PPS 25 ml, CLS-TC 110 ml, CLS-Y 110 ml, SPIN Modules 100 each, Catch Tubes 100 each, User manual 1 each, MSDS 1 each, Certificate of Analysis 1 each
Materials Required but not Supplied
FastPrep® Instrument or alternative high speed disruptor, Microcentrifuge that can freely spin 2.0 ml tubes, Microcentrifuge tubes (2.0 ml and 1.5 ml), Rotator or low-speed vortex
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