Isolation of total RNA from soil supernatants The FastRNA™ Pro Soil-Indirect Kit is designed to efficiently isolate total RNA from organic material found in soil supernatants. Cellular material is washed away from soil particles, centrifuged, and homogenized by any FastPrep® Instrument in impact-resistant 2 mL tubes of Lysing Matrix E. Total RNA is released into a protective solution, extracted, precipitated, and cleaned with the proprietary RNAMATRIX™ and a Quick-Clean Spin Filter. Total RNA prepared with this kit is suitable for RT-PCR analysis and other downstream applications.