Adapted to Sf-900 II SFM. (Cat. No. 10902-088)Sf21 Cells (IPLB-Sf21-AE) were isolated from Spodoptera frugiperda (Fall Armyworm) ovarian insect cells and are used for transient or stable expression of recombinant proteins which saves significant time and expense associated with the adaptation of cultures to serum-free and/or suspension cultures (1). Insect cells have been historically cultured in stationary systems utilizing T-flasks and serum supplemented basal medium (2). Insect cells are generally not anchorage dependent and we recommend that the Sf21 seed stocks be maintained in suspension culture, allowing for more flexibility and ease of use.
Prepared from low-passage cells with documented lineage (100 to 110 total passages and 15 to 20 passages serum-free).
Provided as a frozen stock in Sf-900 II SFM containing 7.5% DMSO.
1.5 x 107 total cells provided.
Can be thawed and used directly in suspension culture for rapid expansion of
cell stocks, propagation of baculovirus stocks, and production of recombinant proteins, or used as a monolayer for transfection or plaque assay applications.
Protocols for recovery, culture, and cryopreservation are provided on the product insert.
Application(s): For baculovirus propagation and recombinant protein expression.Performance testing: Each lot is tested for growth and viability post recovery from cryopreservation. The Master Seed Bank has been tested for viruses, mycoplasma and sterility and identity has been confirmed by isozyme analysis and karyotyping.
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