PLATINUM GENOTYPE TSP DNA POL 2500 UN #Y02028 #95514

• 产品编号：11448032      品牌：invitrogen       原厂货号：11448032
• 产品分类：PCR/RT-PCR/qRT-PCR > PCR > 热启动PCR
• 应用分类：

描述：

Platinum® GenoType Tsp DNA Polymerase is designed and qualified specifically for amplification of dinucleotide repeat markers in PCR-based genotyping applications. The enzyme is pre-complexed with a thermolabile inhibitor containing monoclonal antibodies to Tsp DNA polymerase, providing an automatic "hot-start" method that improves PCR specificity and allows for room temperature reaction assembly. Platinum® GenoType Tsp DNA Polymerase:

 

• Exhibits minimal non-templated nucleotide addition to PCR products
• Lacks both 5´ and 3´ exonuclease activity
• Amplifies fragments up to 500 bpApplication
  Amplification of dinucleotide repeat loci in applications in which elimination of non-templated nucleotide addition is desirableUnit definition
  One unit of Platinum® GenoType Tsp DNA Polymerase has been functionally determined to be equivalent to one unit of Taq DNA Polymerase in amplification of dinucleotide repeats using standardTaq DNA polymerase reaction conditions. One Tsp DNA polymerase unit approximates 2.5 activity units. An activity unit incorporates 10 nmoles of deoxyribonucleotide into acid-precipitable material in 30 min at 74°C under optimized reaction conditions.

• Contents and Storage

  Platinum® GenoType Ts p DNA Polymerase is supplied with 10X PCR Buffer [200 mM Tris-HCl (pH 8.4), 500 mM KCl], and a vial of 50 mM MgCl 2 . Store at -20°C. Guaranteed stable for 6 months when properly stored.

原厂资料：

Platinum® GenoType Tsp DNA Polymerase is designed and qualified specifically for amplification of dinucleotide repeat markers in PCR-based genotyping applications. The enzyme is pre-complexed with a thermolabile inhibitor containing monoclonal antibodies to Tsp DNA polymerase, providing an automatic "hot-start" method that improves PCR specificity and allows for room temperature reaction assembly. Platinum® GenoType Tsp DNA Polymerase:

 

• Exhibits minimal non-templated nucleotide addition to PCR products
• Lacks both 5´ and 3´ exonuclease activity
• Amplifies fragments up to 500 bpApplication
  Amplification of dinucleotide repeat loci in applications in which elimination of non-templated nucleotide addition is desirableUnit definition
  One unit of Platinum® GenoType Tsp DNA Polymerase has been functionally determined to be equivalent to one unit of Taq DNA Polymerase in amplification of dinucleotide repeats using standardTaq DNA polymerase reaction conditions. One Tsp DNA polymerase unit approximates 2.5 activity units. An activity unit incorporates 10 nmoles of deoxyribonucleotide into acid-precipitable material in 30 min at 74°C under optimized reaction conditions.

• Contents and Storage

  Platinum® GenoType Ts p DNA Polymerase is supplied with 10X PCR Buffer [200 mM Tris-HCl (pH 8.4), 500 mM KCl], and a vial of 50 mM MgCl 2 . Store at -20°C. Guaranteed stable for 6 months when properly stored.

注意事项：

For Research Use Only. Not for use in diagnostic procedures.