DYNABEADS MOUSE DC ENRICHMENT 5 ML

产品编号：11429D 品牌：invitrogen 原厂货号：11429D
产品分类：生化和细胞分析 > 微珠和磁珠 > 细胞分选磁珠 > 分离鼠细胞
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包装：

运保温度:

2-8°C

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其它组分：

Depletion MyOne™ Streptavidin Dynabeads®【包装：5 ml】

Antibody Mix containing an optimized mixture of biotinylated monoclonal antibodies towards mouse CD2, CD3ε, CD49b, mIgM and Ter-119【包装：1 ml 】

描述：

Use this kit to enrich viable mouse dendritic cells (DC’s) from spleen/lymph node cells by negative isolation. The kit depletes T cells, mIgM+ B cells, NK cells, erythrocytes and most granulocytes. Highly enriched mouse DC’s are bead and antibody-free, and can be used in any functional assay. The cells can be further sorted to obtain high purity of specific DC sub-populations.

Obtain high recovery (80-100%) of viable Lin-CD11c+ DC’s of all sup-populations, including DC’s positive for CD4, CD8α, B220 and CD19.
Substantial reduction in sorting time and volume compared to sorting only, thus saving money in addition.
Pool cells/tissue for easy sample scale –up

Methodology:
An antibody mix towards the non-dendritic cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining untouched mouse DC’s can be directly sorted into the sup-population of choice or used in any other application.

Starting samples:
Spleen or lymph node cells.