This gentle and easy-to-use kit allows you to isolate flow-compatible human CD8+ T cells from PBMC or directly from whole blood. Immediately after positive isolation, the beads are released and removed from your cell sample. In this way, you avoid exposing your cells to potentially cytotoxic or immunogenic foreign substances like iron oxides or dextrans, a potential problem when working with biodegradable particles. In addition, your cells will not be exposed to the stress of being passed through a column.
You can expect a purity level of >95% human CD8+ cells.
Advantages:
No risk of your results being affected by the isolation method
Gentle tube-based method - no columns needed
High purity and recovery
Bead-free cells - compatible with flow cytometry analysis
Compatible with flow cytometry analysis
Viable, bead-free CD8+ T cells for direct use in any cell-based assay.
The isolated cells have the presumed profile of memory and naive CD8+ T cells, and show a high proliferation capacity. You will save time and money, and get increased confidence in your results.
Starting sample:
MNC or whole blood
Capacity:
The kit will process 2 x 10^9 mononuclear cells (MNC) or 80 ml whole blood
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