The Dynabeads® Untouched™ Human CD8 T Cells Kit is used to isolate pure and viable untouched CD8+ T cells from PBMC by negative isolation. The kit depletes CD4+ T cells, B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes, and erythrocytes. The negatively isolated human CD8+ T-cells are left in the sample and have not been in contact with the Dynabeads®. The bead- and antibody-free CD8+ T cells are truly untouched, and in perfect shape for any functional assay or application.
Advantages of the Dynabeads® Untouched™ Human CD8 T Cells Kit:
• High purity, recovery, and viability of human untouched CD8+ T cells
• Easy to use and to scale up
• No columns required
Magnetic bead-based Separation offers easy handling
An antibody mix towards the non-CD8 cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human CD8+ T cells can be directly analyzed in a flow cytometer and used in any application. For depletion or positive isolation of CD8+ cells, select from our range of Human CD8+ T Cell Isolation Products.
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