DYNABEADS UNTOUCHED HUMAN T CE 10ML, 1X10E9

• 产品编号：11344D      品牌：invitrogen       原厂货号：11344D
• 产品分类：生化和细胞分析 > 微珠和磁珠 > 细胞分选磁珠 > 分离人细胞
• 应用分类：

其它组分：

描述：

Use this kit to isolate pure and viable untouched T cells from PBMC by negative isolation. The kit depletes B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human T cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free T cells are truly untouched, and in perfect shape for any functional assay and application.
 

• High purity, recovery and viability of human untouched T cells
• Easy to use and to scale up
• No columns required

Methodology:
An antibody mix towards the non-T cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human T cells can be directly analyzed in a flow cytometer and used in any application.

Note: A negative isolation strategy isolates pure and truly untouched cells. When maximum cell purity is critical, a positive cell isolation strategy is recommended

Starting Sample:
PBMC

原厂资料：

Use this kit to isolate pure and viable untouched T-cells from PBMC by negative isolation. The kit depletes B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human T-cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free T-cells are truly untouched, and in perfect shape for any functional assay and application.

• High purity, recovery and viability of human untouched T cells
• Easy to use and to scale up
• No columns required

Methodology:
An antibody mix towards the non-T cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human T-cells can be directly analyzed in a flow cytometer and used in any application.

Note: A negative isolation strategy isolates pure and truly untouched cells. When maximum cell purity is critical, a positive cell isolation strategy is recommended

Starting Sample:
PBMC