培养基DMEM-F12，全成分，500ml

产品编号：11330032 品牌：invitrogen 原厂货号：11330032
产品分类：细胞,细胞培养与转染试剂 > 基础培养基 > DMEM⁄F-12营养培养基
应用分类：细胞培养产品与服务 > 哺乳动物细胞培养 > 细胞培养基 > DMEM/F12

包装：

500ML

运保温度:

2-8°C

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描述：

含有 15 mM HEPES 缓冲液和 L-谷氨酰胺。

DMEM ⁄ F-12 是一个广泛使用的基础培养基，支持许多哺乳动物细胞的生长如 MDCK、胶质细胞、成纤维细胞、人内皮细胞、大鼠成纤维细胞等。

DMEM ⁄ F-12配方改良如下：

With

• L-glutamine

• HEPES

• Phenol Red

Gibco® DMEM ⁄ F-12 配方是DMEM 和Ham’s F-12按1:1等体积混合。该配方结合了DMEM培养基含高糖、氨基酸以及F-12培养基含各种维生素的特点。

DMEM ⁄ F-12 不含蛋白质、脂类和生长因子。因此DMEM ⁄ F-12 使用时可能需要加入添加剂，一般是10%FBS。DMEM ⁄ F-12使用碳酸氢钠缓冲系统(2.438 g ⁄ L) 因此在 5-10% CO₂ 环境下培养细胞。

原厂资料：

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM/F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. Life Technologies offers a variety of Gibco® DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM/F-12 is modified as follows:

With

• L-glutamine

• HEPES

• Phenol Red

The complete formulation is available.

Gibco® DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids and vitamins with F-12's wide variety of components.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® DMEM/F-12 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers an identical Gibco® DMEM/F-12 product made in our Scotland facility (31330-095). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system (2.438 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.