Dynabeads® Human T-Activator CD3/CD28用于人T细胞的激活和扩增。
产品优点：
T细胞的激活无需饲养层细胞
激活的细胞仍保留类似体内时的功能
无可溶性抗体或有丝分裂原污染，重复性高
T细胞扩增100-1000倍
原厂资料：
Dynabeads® Human T-Activator CD3/CD28 are for the activation and expansion of human T cells. Advantages of Dynabeads® Human T-Activator CD3/CD28:
• Activation of T cells without the need for feeder cells • Activated cells that retainin vivo-like function • Maximum reproducibility without contamination by soluble antibodies or mitogens • From 100- to 1,000-fold T cell expansion
About Dynabeads® Human T-Activator CD3/CD28 Dynabeads® Human T-Activator CD3/CD28 offer a simple method for activation and expansion of T cells that does not require feeder cells (antigen-presenting cells) or antigen. The uniform 4.5 µm diameter, inert, superparamagnetic beads are similar in size to antigen-presenting cells, and are covalently coupled to anti-CD3 and anti-CD28 antibodies. These two antibodies provide primary and co-stimulatory signals, optimized for efficient T cell activation and expansion. Expansion of the T cell population can be stimulated using recombinant IL-2, and after activation or expansion, the magnetic beads can be easily removed using aDynaMag™ magnet. For expansion of antigen-specific T cells from T cell clones or T cell lines, we recommend usingDynabeads® Human T-Activator CD3/CD28/CD137.
Starting samples Start with mononuclear cells (MNCs)/peripheral blood mononuclear cells (PBMCs) from either whole blood or buffy coat, or T cell subsets including CD3+ T cells, CD4+ or CD8+ T cells, or regulatory T cells.
Downstream applications The activated T cells can be analyzed shortly after activation (for transfection/transduction or to study TCR signaling, proteomics, gene expression, etc.). T cells can be left in culture to differentiate into T helper cell subsets or expansion of polyclonal/antigen-specific T cells.
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