Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM is modified as follows:
With
Without
• Low Glucose
• L-glutamine
• Sodium Pyruvate
• Phenol Red
• HEPES
The complete formulation is available.
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins 查看更多than the original Eagle’s Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g ⁄ L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.
Product Intended Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
cGMP Manufacturing and Quality System
Gibco® DMEM is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers an identical Gibco® DMEM product made in our Scotland facility (11880-028). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g ⁄ L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
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