Platinum® Taq DNA 聚合酶对于 DNA 片段的自动"热启动"扩增来说非常理想,其特异性优于 Taq DNA 聚合酶。 其来源于重组 Taq DNA 聚合酶,制备方法是用含有热不稳定抑制剂的单克隆抗体与 Taq DNA 聚合酶结合。 在 PCR 的最初变性步骤中,抑制剂变性,活性 Taq DNA 聚合酶释放到反应体系中。 Platinum® Taq :
特异性和 PCR 产量优于没有"热启动"的 Taq DNA 聚合酶
PCR 产物最大可达 5 kb
室温配制反应体系(图 1)
Platinum® Blue PCR SuperMix 是含有 Platinum® Taq DNA 聚合酶、dNTP、预混上样/示踪染料、缓冲液、镁和盐的 PCR master mix。 蓝色示踪缓冲液不会影响 PCR 效果。 PCR 循环之后,只需直接将 PCR 产物上样到琼脂糖凝胶上。Platinum® Taq PCRx DNA 聚合酶是 Platinum® Taq DNA 聚合酶,与 PCRx Enhancer System 一起提供,以优化棘手和/或富含 GC 的模板的 PCR 效果。 具有 10X PCRx 扩增缓冲液的 10X PCRx 增强剂溶液 (10X PCRx Enhancer Solution with 10X PCRx Amplification Buffer) 可提供更高的引物特异性、更宽的镁离子浓度范围、更宽的退火温度范围并改善 Taq DNA 聚合酶的热稳定性。应用: 复杂基因组、病毒和质粒模板 DNA 的扩增;以及 RT-PCR (1)。单位定义: 在 74®C 下,一个单位的 Platinum® Taq DNA 聚合酶能在 30 分钟内将 10 nmol 脱氧核糖核酸掺入酸性可沉淀物质。
原厂资料:
Platinum®TaqDNA Polymerase is a convenient and reliable "hot start" thermostable DNA polymerase for PCR that provides enhanced specificity over that ofTaqDNA Polymerase. The "hot start" property of the enzyme preparation is conferred by thermolabile monoclonal antibodies that renderTaqDNA polymerase inactive until the initial PCR denaturation step. Features of Platinum®TaqDNA Polymerase include:
•Convenience—assemble PCR reactions at room temperature •Specificity—"hot start" kinetics reduce nonspecific primer annealing, improving product yield •Product size—use for PCR products up to 10 kb •Flexibility—optional KB Extender gives more versatility in PCR assays
Using Platinum®TaqDNA Polymerase Just as withTaqDNA Polymerase, Platinum®TaqDNA Polymerase has a non-template–dependent terminal transferase activity that adds a 3′ deoxyadenosine to product ends, and has a 5′→3′ exonuclease activity (but not 3′→5′ exonuclease activity). PCR products generated with Platinum®TaqDNA Polymerase may be used in the same downstream applications without protocol modifications. Use Platinum®TaqDNA Polymerase for the amplification of DNA from complex genomic, viral, and plasmid templates, as well as in RT-PCR.
注意事项:
For Research Use Only. Not for use in diagnostic procedures.