Platinum®TaqDNA Polymerase is a convenient and reliable "hot start" thermostable DNA polymerase for PCR that provides enhanced specificity over that ofTaqDNA Polymerase. The "hot start" property of the enzyme preparation is conferred by thermolabile monoclonal antibodies that renderTaqDNA polymerase inactive until the initial PCR denaturation step. Features of Platinum®TaqDNA Polymerase include:
•Convenience—assemble PCR reactions at room temperature •Specificity—"hot start" kinetics reduce nonspecific primer annealing, improving product yield •Product size—use for PCR products up to 10 kb •Flexibility—optional KB Extender gives more versatility in PCR assays
Using Platinum®TaqDNA Polymerase Just as withTaqDNA Polymerase, Platinum®TaqDNA Polymerase has a non-template–dependent terminal transferase activity that adds a 3′ deoxyadenosine to product ends, and has a 5′→3′ exonuclease activity (but not 3′→5′ exonuclease activity). PCR products generated with Platinum®TaqDNA Polymerase may be used in the same downstream applications without protocol modifications. Use Platinum®TaqDNA Polymerase for the amplification of DNA from complex genomic, viral, and plasmid templates, as well as in RT-PCR.
原厂资料:
Platinum®TaqDNA Polymerase is a convenient and reliable "hot start" thermostable DNA polymerase for PCR that provides enhanced specificity over that ofTaqDNA Polymerase. The "hot start" property of the enzyme preparation is conferred by thermolabile monoclonal antibodies that renderTaqDNA polymerase inactive until the initial PCR denaturation step. Features of Platinum®TaqDNA Polymerase include:
•Convenience—assemble PCR reactions at room temperature •Specificity—"hot start" kinetics reduce nonspecific primer annealing, improving product yield •Product size—use for PCR products up to 10 kb •Flexibility—optional KB Extender gives more versatility in PCR assays
Using Platinum®TaqDNA Polymerase Just as withTaqDNA Polymerase, Platinum®TaqDNA Polymerase has a non-template–dependent terminal transferase activity that adds a 3′ deoxyadenosine to product ends, and has a 5′→3′ exonuclease activity (but not 3′→5′ exonuclease activity). PCR products generated with Platinum®TaqDNA Polymerase may be used in the same downstream applications without protocol modifications. Use Platinum®TaqDNA Polymerase for the amplification of DNA from complex genomic, viral, and plasmid templates, as well as in RT-PCR.
注意事项:
For Research Use Only. Not for use in diagnostic procedures.