109007 reacts with the heavy chain of mouse IgG2b. 109007 has been cross-absorbed against mouse IgM, IgG1, IgG2a, IgG3 and IgA, pooled human sera and purified human paraproteins. The antibody shows minimal crossreactivity with human immunoglobulins.
Species Cross-Reactivity
Target Species
Cross Reactivity
Human
No
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Flow Cytometry
Neat - 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106cells in 100ul.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from the date of despatch.
Antiserum Preparation
Antiserum to mouse IgG2b was raised by repeated immunisation of goats with highly purified antigen. The preparation was purified by affinity chromatography on mouse IgG2b covalently linked to agarose.
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