最受欢迎,最容易使用的Ladder 1 Kb Plus DNA ladder 是我们最受欢迎的DNA ladder,用于估计PCR产物,酶切产物以及其他的分子生物学实验操作过程中产生的大多数双链DNA条带的分子量。与其他DNA ladder 不同的是, 1 Kb Plus DNA ladder 专门设计为have bands of round, 便于记住条带大小. 定位条带的位置以及便于识别的双重线能够帮助您迅速估计您感兴趣条带的大小。1 Kb Plus DNA ladder 的迁移模式(migration pattern)可在下图查看(图 1).
一种Ladder就可以满足您的大多数需求 1 Kb Plus DNA ladder 由 20 个大小从 100 至 12000 bp 的双链DNA条带组成。它的宽范围使它成为最常用的DNA ladder之一 . 它特别适合于克隆应用和终点PCR(end point PCR)以及其他分子生物学应用
简单的形式适用于所有DNA琼脂糖凝胶 1 Kb Plus DNA ladder 与所有的琼脂糖凝胶(包括预制琼脂糖凝胶和E-Gel等产品)兼容,可用来分析DNA片段,该ladder是以纯化的形式提供的,没有预混液。在加入琼脂糖凝胶之前需要与合适的缓冲液混合。 1 Kb Plus DNA Ladder 提供了 1 µg/µl in 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, and 1 mM EDTA. Store at -20°C, r最佳的液态储存环境,从而避免反复冻融。
DNA染色与放射性标记相容 可通过SYBR®,溴化乙锭或其他的DNA染料染色后观察1 Kb Plus DNA。. 1 Kb Plus DNA 可以通过T4多核苷酸激酶,T4 DNA聚合酶,DNA聚合酶 I,或者其他的DNA聚合酶 I 的大片段(Klenow 片段)来进行放射性同位素标记。. 可以使用任何放射性dNTP进行放射性标记.
仅研究使用,不能用于任何动物或人类治疗或诊断。
原厂资料:
1 Kb Plus DNA ladder is a powerful tool for estimating the molecular weight of linear, double stranded DNA fragments. 1 Kb Plus DNA ladder is composed of 20 highly purified, double stranded DNA bands spanning 100 base pairs to 12,000 base pairs. 1 Kb Plus DNA ladder has 12 evenly spaced bands ranging from 1 Kb to 12 Kb, a quick orientation band at 1650 base pairs that forms a distinct doublet with the 2 Kb band, and seven bands of round sizes below 1 Kb. 1 Kb Plus DNA ladder is compatible with agarose gels you cast in your lab as well as E-Gel® pre-cast agarose gels.
Round, easy to remember band sizes
Broad size range (100bp – 12Kb)
Compatible with all agarose gels
Our Most Popular and Easiest to Use Ladder
1 Kb Plus DNA ladder is our most popular DNA ladder used to estimate molecular mass of double stranded DNA bands from PCR, restriction digests and other molecular biology protocols. Unlike some other DNA ladders, 1 Kb Plus DNA ladder has been specially designed to have bands of round, easy to remember sizes. The logical band lay out, and quick identification doublet, allow you to quickly and easily estimate the size of your band of interest at a glance. The migration pattern of 1 Kb Plus DNA ladder can be found in the image below (Fig 1).
A Single Ladder for Most Your Needs
注意事项:
For Research Use Only. Not for use in diagnostic procedures.