1065002L recognises human ZAP-70, a 70kDa tyrosine protein kinase which associates with the T-cell receptor (TCR) zeta chain and undergoes phosphorylation following TCR stimulation. ZAP-70 is primarily expressed in T lymphocytes and natural killer cells, where it plays a key role in T-cell receptor (TCR) signalling. Recent studies suggest that ZAP-70 is also expressed in a population of normal immature B-cells and in B-cells from a sub-set of patients with chronic lymphocytic leukaemia.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Flow Cytometry(1)
(1)
Membrane permeabilisation is required for this application. Serotec recommends the use of Leucoperm™(Product CodeBUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. This product should be stored undiluted.
This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
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