Plasmid pCMV·SPORT-bgal is used as a positive control for
monitoring expression in eukaryotic cells. The plasmid contains the
reporter gene b-galactosidase (b-gal) from E. coli cloned as a Not I
fragment into plasmid pCMV·SPORT1. The plasmid also contains
a CMV promoter upstream of the b-gal gene, followed by the SV40
t-intron and polyadenylation signal. The b-lactamase gene allows
selection for ampicillin resistance in E. coli.
Promoter:
CMV
Protein Tag or Fusion:
Untagged
Reporter:
Beta-Gal (lacZ)
Constitutive or Inducible System:
Constitutive
Delivery Method:
Transfection
Key Function:
Constitutive Expression,
Reporter Assays
Selection Agent (Eukaryotic):
None
Vector Type:
pCMV
Product Size:
25 µg
Regulatory Statement:
For Research Use Only. Not for use in diagnostic procedures.
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