105005 recognises mouse kappa light chains, both bound and free as shown by ELISA.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/4000 - 1/8000
Flow Cytometry
Immunoblotting
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Antiserum Preparation
105005 was made from pooled antisera from goats immunised with mouse paraproteins bearing kappa light chains. Antiserum was purified by affinity chromatography on pooled mouse Igs with kappa light chains. The antiserum was cross adsorbed on pooled mouse Igs with lambda light chains.
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