The 50 bp DNA Ladder is suitable for sizing double-stranded DNA from 50 to 800 bp. Prepared from a plasmid containing repeats of a 50 bp DNA fragment, the ladder consists of 16 bluntended fragments ranging in length from 50 to 800 bp, at 50-bp increments, and vector fragments >2,000 bp. The double-stranded ladder can be visualized on 2% to 3% agarose gels after ethidium bromide staining. For easy reference on agarose gels, the 350 bp band is two- to three-times brighter than the other bands in the ladder. This ladder can be stained with ethidium bromide or radiolabeled using T4 polynucleotide kinase or T4 DNA polymerase.
原厂资料:
The 50 bp DNA Ladder is suitable for sizing double-stranded DNA from 50 to 800 bp. Prepared from a plasmid containing repeats of a 50 bp DNA fragment, the ladder consists of 16 bluntended fragments ranging in length from 50 to 800 bp, at 50-bp increments, and vector fragments >2,000 bp. The double-stranded ladder can be visualized on 2% to 3% agarose gels after ethidium bromide staining. For easy reference on agarose gels, the 350 bp band is two- to three-times brighter than the other bands in the ladder. This ladder can be stained with ethidium bromide or radiolabeled using T4 polynucleotide kinase or T4 DNA polymerase.