Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Life Technologies offers a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This MEM is modified as follows:
With
Without
• L-glutamine
• HEPES
• Gentamycin
• Phenol Red
• Proline
• Insulin
• Sodium pyruvate
• Zinc
Gibco® Richter’s MEM is a modified formulation of Eagle's MEM that contains 查看更多iron, zinc, putrescine, fatty acids, and gentamycin. Richter’s modification also includes very high concentrations of both choline chloride and i-Inositol.
Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
cGMP Manufacturing
Gibco® MEM is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards.
Gibco® MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g ⁄ L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.