102011S recognises the heavy chain of mouse IgM as demonstrated by ELISA and flow cytometry. Minimal cross reactivity is observed with human immunoglobulins.
102011S has been cross absorbed against Mouse IgG1, IgG2a, IgG2b, IgG3and IgA, pooled human sera and purified human paraproteins.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Neat - 1/5
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106cells in 100ul.
Storage
+4ºC DO NOT FREEZE
This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
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