102009 recognises the heavy chain of mouse IgM as demonstrated by ELISA and flow cytometry. Minimal cross reactivity is observed with human immunoglobulins.
102009 has been cross absorbed against Mouse IgG1, IgG2a, IgG2b, IgG3and IgA, pooled human sera and purified human paraproteins.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Purified Ig prepared by affinity chromatography on IgM covalently linked to agarose
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