Recombinant protein G is produced in a strain of E. coli which contains the gene for protein G from Streptococcus sp. This product is prepared by covalently coupling highly purified rec-Protein G to Sepharose® 4B. It is supplied as a 50% suspension containing 2 ml rec-Protein G-Sepharose® 4B + 2 ml PBS (10 mM phosphate-buffered saline, pH 7.4, containing 0.1% Proclin preservative).
Applications: Immunoglobulin affinity purification and Immunoprecipitation.
Recombinant protein G binds to the Fc region of many types of immunoglobulin G including:
• all four subclasses of human IgG • mouse IgG • rabbit IgG • guinea pig IgG • rat IgG • goat IgG • sheep IgG • cow IgG • horse IgG
Recombinant protein G does not bind to IgG from chicken or cat, or to human IgA, IgM, or serum albumin. It binds weakly to IgG from dog.
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注意事项:
For Research Use Only. Not for use in diagnostic procedures.