This product is supplied as a 50% suspension (5 ml Protein A-Sepharose® 4B + 5 ml PBS) in 10 mM phosphate-buffered saline, pH 7.4,
containing 0.1% Proclin as a preservative. The product is prepared by covalently coupling highly purified (>98% by SDS-PAGE) Staph.
Aureus Cowan strain 1 Protein A to Sepharose® 4B
Protein-A/Sepharose® ratio: ~2.7 mg Protein A per ml beads
Binding capacity: ~22 mg Rabbit IgG per ml beads
Protein A Molecular Weight: 42 kDa (native)
Protein A Ig Binding Sites: 4 per molecule