This kit provides a faster and easier solution for your immunoprecipitation experiments than Sepharose or Agarose, and includes all reagents required to perform small scale immunoprecipitation (IP) using your own antibody (Ab) of choice. The kit is optimal for standard IP, as well as co-IP and ChIP. The kit contains the widely used Dynabeads® Protein G and include all required buffers for immunoprecipitation. When using this kit, you will:
Reduce your protocol time to <30 minutes.
Decrease or even eliminate background caused by non-specific binding - Easy removal of all supernatant after incubation and washing, with no risk of sample loss
Minimize antibody usage and maximise target protein recovery - Rapid binding kinetics and high capacity
Keep your protein complexes intact - Gentle magnetic separation and rapid binding kinetics avoid physical stress on your precious proteins
Improve reproducibility - Consistent high performance of
Increase confidence in results - Optimised and reproducible buffer compositions
Applications:
Dynabeads® Protein G is widely used for immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), small scale antibody purification and other protein isolations.
The magnetic separation technology is fast and gentle, with no centrifugation, rapid binding kinetics and short protocol time. Hence your precious target proteins will experience minimal physical stress. This permits the isolation and concentration of labile complexes that might otherwise dissociate or be damaged by proteases during long incubation times. Native protein conformation and intact, large protein complexes will be preserved.
Benefits and Features:
Superior signal-to-noise properties
Highly reproducible results
No columns, centrifugations, or time-consuming pre-treatment of your samples
Gentle, minimal physical stress on precious proteins
Easy handling and simple, short protocol.
The product holds reputable Dynal high standards with respect to reproducibility and automation ability, and drives reliability for your assays.
Binding capacity:
Approx. 250 µg hIgG per ml (30 mg) Dynabeads®
Dynabeads® Protein G can be used with most antibodies (Ab). (See table for ‘Binding strength’ in the Images and Data section below for specific affinities of Ab from different species and subclasses). The amount of Ab captured is dependent on the Ab concentration in the starting sample and the type and source of the Ab.
Starting samples:
Typically cell lysate, plasma, serum, tissue culture, hybridoma supernatants or similar.
Additional Info:
Dynabeads® Protein G and Dynabeads® Protein A are also available separately separately (without buffers), both as research products for end-users and in larger volumes for OEM supply.
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