(PBS Tablets)
Without calcium and magnesium
1 Tablet = 100 ml
Directions for Tablets:
Dissolve 1 tablet in 100 ml distilled water. Autoclave for 10 minutes @ 115oC. The pH will be in the range 7.3 - 7.5. The buffer can be adjusted as desired with dilute HCl or NaOH.
Formulation:
Component
mg/lt
Mol. Wt.
Mol. (mM)
Inorganic Salts
Potassium Chloride [KCl]
200.00000
74.55
2.68
Potassium Phosphate Monobasic [KH2PO4]
200.00000
136.09
1.47
Sodium Chloride [NaCl]
8000.00000
58.44
136.89
Sodium Phosphate Dibasic [Na2HPO4]
1150.00000
141.96
8.10
Availability:
1X Liquid:
Earle's Balanced Salts (EBS), Modified:
Catalog Number
Description
Size
1800049
1X EBS (Modified)
100 ml
1800054
500 ml
1800254
1X EBS (Modified) without phenol red
500 ml
1800449
1X EBS (Modified), without calcium, magnesium
100 ml
1800454
500 ml
1900154
10X EBS (Modified), without sodium bicarbonate
500 ml
1700122
Powdered, EBS (Modified), without sodium bicarbonate
1 x 10 liter
Phosphate Buffered Saline (PBS):
Catalog Number
Description
Size
1860049
1X PBS
100 ml
1860054
500 ml
1860449
1X PBS without calcium, magnesium
100 ml
1860454
500 ml
1861054
1X PBS with magnesium chloride, without magnesium sulfate
500 ml
Hanks' Balanced Salts (HBS), Modified:
Catalog Number
Description
Size
1810049
1X HBS (Modified)
100 ml
1810054
500 ml
1810254
1X HBS (Modified) without phenol red
500 ml
1810449
1X HBS (Modified), without calcium, magnesium
100 ml
1810454
500 ml
1810554
1X HBS (Modified), without calcium, magnesium, phenol red
500 ml
10X Liquid:
Earle's Balanced Salts (EBS), Modified:
Catalog Number
Description
Size
1900154
10X EBS (Modified), without sodium bicarbonate
500 ml
Phosphate Buffered Saline (PBS):
Catalog Number
Description
Size
1960054
10X PBS
500 ml
1960449
10X PBS without calcium, magnesium
100 ml
1960454
500 ml
1961054
10X PBS with magnesium chloride, without magnesium sulfate
500 ml
Hanks' Balanced Salts (HBS), Modified:
Catalog Number
Description
Size
1910149
10X HBS (Modified) without sodium bicarbonate
100 ml
1910154
500 ml
1910349
10X HBS (Modified) without sodium bicarbonate, phenol red
100 ml
1910654
10X HBS (Modified), without calcium, magnesium, sodium bicarbonate
500 ml
Powders:
Earle's Balanced Salts (EBS), Modified:
Catalog Number
Description
Size
1700122
Powdered, EBS (Modified), without sodium bicarbonate
1 x 10 liter
Phosphate Buffered Saline (PBS):
Catalog Number
Description
Size
1760020
PBS, powder, with calcium, magnesium
10 x 1 liter
1760022
1 x 10 liter
1760420
PBS, powder, without calcium, magnesium
10 x 1 liter
Hanks' Balanced Salts (HBS), Modified:
Catalog Number
Description
Size
1710120
HBS (Modified), powder, without sodium bicarbonate
10 x 1 liter
1710122
1 x 10 liter
1710124
1 x 50 liter
Tablets:
Phosphate Buffered Saline (PBS):
Catalog Number
Description
Size
2810301
2810305
2810306
2810307
PBS tablets, without calcium, magnesium (1 tablet makes 100 ml)
10 tab (sample)
100 tab
200 tab
500 tab
References:
Dulbecco, R., Vogt, M., 1954,J. Exp. Med.,v. 99: 167
Paul, J., 1959,Cell and Tissue Culture, Livingstone, LTD., London