MOUSE ANTI HUMAN ANGIOGENIN

产品编号：0555-5008 品牌：AbD Serotec 原厂货号：0555-5008
产品分类：抗体 > 一抗 > 蛋白特异性一抗
应用分类：

包装：

0.15 mg

运保温度:

+4oC or -20oC

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描述：

Specificity

Mouse anti Human Angiogenin (MANG-1) is a monoclonal antibody specific for angiogenin, a potent angiogenesis inducing polypeptide of approximately 16.5kDa responsible for inducing vascularization in both normal and malignant tissues through activation of vascular endothelium and smooth muscle cells ant triggering a number of processes including Cell migration and proliferation (Reviewed in Gao et al.2008) Angiogenin, also known as ribonuclease 5 demonstrates tRNA specific ribonuclease activity (Saxena et al. 1992), this in turn is regulated by binding to the ribonuclease inhibitor RNH1 (Tuefel et al. 2003) . Angiogenin exerts its vascular stimulation potential through binding to cytoplasmic actin on the surface of endothelial cells, followed by endocytosis and subsequent translocation to the nucleus

Mouse anti Angiogenin, clone MANG1 stains single cells on human tonsil sections and endothelial cells in human terminal placenta. MANG-1 also demonstrates intense staining on carcinoma cells and of endothelial cells in intratumoral vessels (Marioni et al. 2010)

Application

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.

Application Name	Yes	No	Not Determined	Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen				1/60
Immunohistology - Paraffin⁽¹⁾				1/30
Immunoprecipitation
Western Blotting

(1)

Proteinase K pretreatment for antigen retrieval is recommended for use with paraffin sections.SeeMarioni et al. 2011

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Storage

Prior to reconstitution store at +4^oC.
After reconstitution store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.