0500-0100 will recognise both the heavy and light chains of the F(ab')2 portion of human IgG by IEP. This product will also react with light chains from other human immunoglobulin types. This antibody has been cross adsorbed against the Fc domain and will react with less than 1 % of the Fc domain of human IgG.
This alkaline phosphatase-labelled antibody is a performance tested, matched reagent for AbD Serotec HuCAL antibodies.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/1,000 - 1/10,000
Western Blotting
1/1,000 - 1/10,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Storage
Stable for 6 weeks at 4ºC as an undiluted liquid. For longer term storage (>6 weeks) please add an equal volume of glycerol and store at -20ºC. Avoid freeze-thaw cycles.
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