FO GSL is a glycosphingolipid differentiation antigen that is present on macrophages in the red pulp of the spleen, and the medulla of inguinal and axial lymph nodes. FO GSL is also present on reticular cells in the T-cell area of the spleen and lymph nodes. A subpopulation of FOM-1 positive macrophages also appear in the peritoneal cavity in response to inflammatory stimuli. Monocytes, granulocytes and other macrophages in lymphoid and non-lymphoid organs are not stained by FOM-1.
This antibody also recognises a marker present on sheep erythrocytes.
Species Cross-Reactivity
Target Species
Cross Reactivity
Sheep
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Flow Cytometry
Immunohistology - Frozen
1/100 - 1/400
Immunohistology - Paraffin
1/100
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
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