0480-0404P recognises salivary amylase, formally known as ptyalin, an alpha-amylase digestive enzyme and member of the glycosyl hydrolase 13 family, which breaks down insoluble starch into soluble starches (dextrins) and maltose.
0480-0404P also recognises porcine amylase (50%) and weakly fungal (Aspergillus spp.) amylase (10%).
Species Cross-Reactivity
Target Species
Cross Reactivity
Fungal
Yes
Pig
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/50 - 1/500
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human salivary amylase was raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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