0220-1004P recognises bovine Albumin (BSA), a soluble protein which constitutes approximately 50% of blood serum protein, acting as a regulator of blood colloidal osmotic pressure and hence blood volume. Albumin is also an important transporter of substances within the bloodstream, such as fatty acids, thyroid hormones, metal ions and steroids.
BSA is produced as a by-product of the beef industry and can be used as a nutrient in cell cultures, as an enzyme stabilizer during DNA digestion, and as a diluent or blocking agent in immunohistochemistry and Enzyme-Linked Immunosorbent Assays (ELISAs).
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/100 - 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to bovine albumin were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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