苯甲基磺酰氟(PMSF)Phenylmethylsulfonyl Fluoride

产品编号：0219538101 品牌：MP 原厂货号：0219538101
产品分类：生化试剂 > 生化试剂 > 常用化学试剂
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包装：

1 g

运保温度:

2-8°C

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描述：

Crystalline
Purity: >99%
Protease inhibitor. A relatively safe substitute for toxic di-isopropylfluorophosphate. Inhibits mammalian acetyl cholinesterase, but not those from fish sources.
Ref.: 1. Fahrney, D.E. and Gold, A.M., J. Am. Chem. Soc., 85: 997 (1963). 2. Moss, D.E. and Fahrney, D.E., Biochem. Pharmacology, 27: 2693 (1978). 3. Turini, P., et al., J. Pharmacol. Exp. Ther., 167: 98 (1969).

Description: An irreversible inhibitor of serine proteases - inhibits trypsin, chymotrypsin and protease. Causes sulfonylation of the active-site serine residue. A less toxic substitute for the extremely toxic diisopropylfluorophosphate. Inhibits mammalian acetyl cholinesterase, but not those from fish sources. Recommended concentration is 15 to 175 ug/ml.

苯甲基磺酰氟用途　　生化研究。与羰基化合物缩合制备烯烃。胰蛋白酶、胰凝乳蛋白酶的抑制剂，可抑制丝氨酸蛋白酶例如胰蛋白酶(trypsin)和胰凝乳蛋白酶(chymotrypsin)，也可抑制半胱氨酸蛋白酶和乙酰胆碱酯酶。有效浓度为0.1-1mM。

注意事项：

Working solutions must be prepared fresh and added at several steps during sample preparation. Half-life of 1 hour at pH 7.5. Stock solutions of 200 mM or higher in anhydrous solvent are stable for at least 9 months at 4°C.