3-(N-吗啡啉)丙磺酸（MOPS）MOPS

• 产品编号：0219483780      品牌：MP       原厂货号：0219483780
• 产品分类：生化试剂 > 生物缓冲液 > MOPS & MOPSO
• 应用分类：核酸分离纯化与分析 > 核酸凝胶电泳与印迹 > RNA电泳 > Denaturing RNA Electrophoresis

描述：

3-[N-Morpholino]propanesulfonic acid
Molecular Biology Reagent
Purity: 99+%
Free Acid
Useful buffer range: 6.5-7.9

Description: MOPS is a zwitterionic amino acid which acts as one of the "Good" buffers.¹ It is a structural analog to MES; however, MOPS is a more suitable biological buffer than MES because the pH range is closer to pH 7.4.

A buffer using MOPS free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pK_a ± 1), using about a half-equivalent of NaOH. A buffer using MOPS sodium salt can be made by mixing equimolar MOPS free acid and MOPS sodium salt solutions to attain a buffer of the desired pH. Titration of a solution of MOPS sodium salt with HCl results in a solution that will contain NaCl, so the ionic strength will be higher than appropriate for some applications.

Concentrations higher than 20 mM should not be used with mammalian cell cultures.

3-(N-吗啡）丙烷磺酸；3-(N-吗啉基）丙磺酸
用途：生物缓冲液

适用于叶绿体薄层备样的电子传递和磷酸化研究；可以配制成多种琼脂培养基，在极限培养基中作为无毒缓冲液应用于棒状链霉菌（Streptomyces clavuligerus）的培养和头孢菌素（cephalosporin）的生产；可作为二维凝胶电泳中等电聚焦电泳（IEF）的电解质系统成分；还可应用于Northern杂交，作为RNA的分离和转膜时的缓冲液。

名称：3-(N-吗啡啉)丙磺酸 MOPS（有配好的缓冲液）

   英文：MOPS 3(N-MORPHOLINO)PROPANE-SULFONIC ACID

   CAS：1132-61-2

   介绍：MOPS是一种两性离子缓冲液，主要用用于配制作RNA 变性电泳缓冲液，缓冲范围是：6.5-7.9。在20mM的甲醛胶中作用尤为出色。多酚氧化酶（PPO）的活性测定会用到。

原厂资料：

注意事项：

The pH of a 0.1 M solution of the sodium salt is approximately 10-12 and a 0.1 M solution of the free acid is approximately pH 2.5 to 4.0, depending on temperature. Solutions can be stored refrigerated for tup to approximately 6 months. Solutions should not be autoclaved¹; sterilization of solutions should be done by filter sterilization through a 0.2 um filter.