甘氨酸Glycine

• 产品编号：0219482594      品牌：MP       原厂货号：0219482594
• 产品分类：细胞,细胞培养与转染试剂 > 添加剂 > 氨基酸
• 应用分类：蛋白质研究 > 蛋白质凝胶电泳
  　　　　　暂定分类 > 通用培养基添加剂 > 氨基酸溶液Amino Acid Solution

描述：

Glycine
Catalog Number: 100570, 194049, 194681, 194825, 808822, 808831
Molecular Biology Reagent
Purity: 99+%
Ideal for all molecular biology applications and buffer preparations.
Synonyms: Aminoacetic acid; Glycolixir; Hampshire Glycine

Structure:



Molecular Formula: C₂H₅NO₂

Molecular Weight: 75.07

CAS #: 56-40-6

Synonyms: Aminoacetic acid; Gly; Aminoethanoic acid; Glycocoll; G

Solubility: Soluble in water (250 mg/ml @ 25°C; 672 mg/ml @ 100°C); slightly soluble in alcohol and ethanol(95%); almost insoluble in ether.¹

Description: A non-essential amino acid. Only amino acid with no asymmetric carbon.¹ Major inhibitory neurotransmitter.¹ Commonly used as a component in Tris-glycine and Tris-glycine-SDS running buffers for polyacrylamide gel electrophoresis^6,7,8, a component of Towbin's transfer buffer for Western blots¹⁰, a buffer substance in cryoenzymology⁵, in osmotic pressure maintenance in isoelectric focusing of erythrocytes⁹, salting-in effect in protein chemistry³, and as a buffer component in the coupled phosphatase-kinase reaction for end labelling of restriction fragments.⁴ The growth requirements of various microorganisms is reported in the Handbook of Microbiology.²

Availability:
 

References:
 

1. Merck Index, 12th Ed., No. 4500.
2. CRC Handbook of Microbiology, Laskin, A.I. and Lechevalier, H.A. (eds.), v. 4, 1, CRC Press: Cleveland, OH (1974).
3. Arakawa, T. and Timasheff, S.N., Meths. Enzymol., v. 114, 49 (1985).
4. Cobianchi, F. and Wilson, S.H., Meths. Enzymol., v. 152, 101 (1987).
5. Fink, A.L., Geeves, M.A., Meths. Enzymol., v. 63, 336 (1979).
6. Hames, B.D. and Rickwood, D., Gel Electrophoresis of Proteins: A Practical Approach, 2nd ed., IRL Press: New York, New York, p. 32-35 (1990).
7. Jagus, R., Meths. Enzymol., v. 152, 296 (1987).
8. Laemmli, U.K., "Cleavage of structural proteins during the assembly of the head of bacteriophage T4." Nature, v. 227, 680-685 (1970).
9. McGuire, J.K., et al., J. Chromatogr., v. 194, 323 (1980).
10. Towbin, H., et al., "Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications." Proc. Natl. Acad. Sci. USA, v. 76, 4350-4354 (1979).

11. 用途说明

    　　用作络合滴定指示剂，色层分析用试剂；缓冲剂；比色法测定氨基酸时作标准用。检验铜、金和银。制备组织培养基。在有机合成和生物化学中用作生化试剂和溶剂。
     
    　　脊髓抑制性神经递质，NMDA受体的变构调节
     
    　　分子生物学级用做缓冲液组分，在耦联磷酸激酶反应中用于末端标记限制性酶切片段；Tris -甘氨酸缓冲液组分，用于SDS -聚丙烯酰胺凝胶电泳中的体外翻译产物应用中。

原厂资料：

• Western blot