3-[3-(胆酰胺丙基)二甲氨基]丙磺酸内盐（CHAPS）CHAPS

产品编号：0219031901 品牌：MP 原厂货号：0219031901
产品分类：生化试剂 > 化学试剂 > 表面活性剂（增溶剂和去污剂） > 其它
应用分类：蛋白质研究 > 蛋白质纯化 > 标签蛋白亲和纯化 > 生物素亲和纯化

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描述：

3-[(3-Cholamidopropyl)-dimethyl-ammonio]-1-propane sulfonate
A nondenaturing zwitterionic detergent for solubilizing membrane proteins.
Ref.: Hjelmeland, L.H., Proc. Nat. Acad. Sci. USA, 77: 6368 (1980).

Description: A nondenaturing zwitterionic detergent for solubilizing membrane proteins and breaking protein-protein interactions.^1,11 Combines the useful properties of both the sulfobetaine-type and the bile salt detergents. Commonly used for protein solubilization in isoelectric focusing and two-dimensional electrophoresis^6,13, especially for non-denaturing (without urea) isoelectric focusing. CHAPS has been shown to give excellent resolution of some subcellular preparations^2,12 and plant proteins.⁸ Concentrations between 1-4% (v/v) are typically used in an isoelectric focusing gel.^3,4 A commonly used isoelectric focusing sample solution consists of 8 M urea, 4% CHAPS, 50-100 mM dithiothreitol (DTT) and 40 mM Tris.⁴ Its small micellar molecular weight (6150) and high CMC (6-10 mM) allow it to be removed from samples by dialysis. Can be used as a non-toxic stabilizing agent for growth factors.

3-[(3-胆固醇氨丙基)二甲基氨基]-1-丙磺酸

用途：两性表面活性剂，两性离子去垢剂、蛋白质裂解液，用于增溶膜蛋白和裂解蛋白-蛋白之间的相互作用。CHAPS分子结构中具有一个胆酸和硫代甜菜碱，在紫外区的低吸光性成为用紫外方法检测膜蛋白的首选去垢剂，CMC值均为8mM，常用于于双向电泳等实验。
原理：CHAPS保护蛋白质的天然状态，可溶解膜蛋白，从而解除蛋白与蛋白质间的相互作用。透析可除去。

注意事项：

When preparing solutions of CHAPS, it is preferable to avoid stirring and/or shaking. For a 10% solution, i.e., 1 g CHAPS solid into beaker followed by 9 g of water. Cover beaker with watch glass and allow it to sit at room temperature for 30-60 minutes. Solutions of up to 1 M (60%) can be made in this way.