Product Description5-Bromo-4-chloro-3-indolyl b-D-galactopyranoside, commonly known as X-Gal, is a histochemical substrate for b-galactosidase. X-Gal is cleaved by b-galactosidase to yield an insoluble blue precipitate. X-Gal is particularly useful in molecular biology applications to detect the activity of b-galactosidase which is frequently used as a reporter gene. Insertion of DNA into the lac Z region results in the loss of b-galactosidase activity. Lac+ bacterial colonies resulting from a-complementation will appear blue whereas bacterial colonies containing plasmid with DNA inserted in the lac Z region, will be incapable of a-complementation and will appear white.
Applications
5-Bromo-4-chloro-3-indolyl b-D-galactopyranoside is used as indigogenic substrate for b - galactosidase, for detection of b - galactosidase-positive clones, and the identification of lac and bacterial colonies or phage plaques.
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