Synonyms Bile salt activated lipase; sterol esterase; carboxyl ester lipase; steryl-ester acylhydrolase; Cholesterase; Neutral cholesteryl ester hydrolase (http://druginfo.nlm.nih.gov/drugportal/ProxyServlet?mergeData=true&objectHandle=DBMaint&APPLICATION_NAME=dru
Pseudomonas species, ≥100 u/mg solid (Activity)
Excess cholesterol is stored intracellularly as cholesterol esters. Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols.
ApplicationsCholesterol Esterase is useful for enzymatic determination of total cholesterol when coupled with cholesterol oxidase in clinical analysis.
Unit Definition
One unit is the amount of enzyme that will hydrolyze 1.0 micromole of cholesterol ester per minute at 37 °C, pH 7.0.
NOTES
Stabilizers: Mg- - Na-cholate, bovine serum albumin; Inhibitors: Hg- - Ag ionic detergents; Michaelis constants:5.4 x 10-5M(Linoleate), 6.6 x 10-5M(Oleate); 3.7 x 10-5M(Lnolante), 1.5 x 10-5M(Palmitate); 1.2 x 10-5M(Myristate), 2.3 x 10-5M(Stearate); The