Tricine三甲基甘氨酸（Tricine）

• 产品编号：0210311225      品牌：MP       原厂货号：103112
• 产品分类：生化试剂 > 生物缓冲液 > Bicine & tricine
• 应用分类：蛋白质研究 > 蛋白质凝胶电泳
  　　　　　暂定分类 > 膜蛋白表达 > SDS-PAGE > SDS-PAGE Buffers & Reagents

描述：

N-Tris-[hydroxymethyl]methylglycine; N-[2-Hydroxy-1,1-bis(hydroxymethyl) ethyl] glycine
Electrophoresis Grade
Purity: >99%
Heavy metals (as Pb): <10 ppm
pKa at 25°C = 8.1
Biological buffer

Description: Tricine was first prepared by Good for use as a buffer for chloroplast reactions. It is structurally similar to Tris, but is much less inhibitory at high concentrations.⁵ For ATP assays using firefly luciferase, tricine buffer at 25 mM was found to be the best of ten common buffers tested.¹⁰
Tricine can be used in cryopreservation medium for the preservation of tissues and organs. Cryopreservation depends on the physical and chemical characteristics of the preservation medium used. The pH values and pK values for tricine/DMSO mixtures has been reported down to -20°C.⁸

Tricine has been found to be an efficient scavenger of hydroxyl radicals in a study of radiation-induced membrane damage.⁷
Tricine is typically the buffer of choice in SDS-PAGE systems when separating proteins in the range of 1 to 100 kDa.⁹

Typical Preparation: A buffer may be prepared by titrating with sodium hydroxide to the desired pH, using about a half-equivalent of NaOH.

 别名：三(羟甲基)甲基甘氨酸 ;N-三(羟甲基)甲基甘氨酸，N-三-(羟甲基)甲基氨基氨基乙酸
用途：生物缓冲剂，是一种高效、广谱防腐剂，能够有效抑制革兰氏阳性菌、革兰氏阴性菌、酵母菌及霉菌。可用于香波、冷烫剂、染发剂、调理剂、香皂、洗发膏等碱性化妆品及洗涤剂。

原厂资料：

注意事项：

Metal Binding Constants (log K) for 0.1 M solution at 20°C:²
 

• Mg2+: 1.2
  Ca2+: 2.4
  Mn2+: 2.7
  Cu2+: 7.3

• Western blot