This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Immunohistology
This antibody is suitable for use with formalin fixed, paraffin embedded sections and gives a strong reaction at 1/20 dilution. This product does not require antigen retrieval. The recommended incubation time is 35 minutes.
Western Blotting
0200-0423 detects a major band of approximately 50 kDa with minor bands at 35 kDa, 40 kDa and 67 kDa. This reflects bands found in DTT reduced blots of the immunising antigen and in immunoblots using a known antibody positive serum.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
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