0151-9104 recognises numerous Adeno proteins including hexon protein, and reacts with Adenovirus types 1, 2, 3, 5, 6, 7a, 8, 31, 40 & 41. Broad reactivity with other Adenovirus types is expected. This antibody does not cross-react with Parainfluenza types 1-3, Influenza A & B, RSV, hMPV and Rhinovirus type 16. It does not react with uninfected Hep-2, LLCMK2 and Vero cells.
Adenoviruses are 80-110 nm diameter, non-enveloped icosahedral viruses that can cause respiratory illness, infantile gastroenteritis and other mild disorders such as conjunctivitis. Features of Adenovirus have made it a useful tool for gene transfection and expression in mammalian cells.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/5,000
Immunofluorescence
1/8,000
Western Blotting
1/1,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
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