0100-0662B detects the alpha subunit of Follicle-stimulating hormone (FSH alpha). FSH is secreted by the pituitary, and is a member of the glycoprotein hormone family which includes Human chorionic gonadotropin (hCG), Luteinizing hormone (LH), and Thyroid stimulating hormone (TSH).
These hormones are all structurally related and contain a common alpha subunit non-covalently bound to a hormone specific beta subunit, which determines receptor specificity. Both of the subunits are necessary for hormone action.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/100 - 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
0100-0662B is suitable for use in a two site assay with0100-0663bound to the solid phase and this antibody for detection. When tested in a sandwich ELISA the 50% OD was observed at an antibody dilution of 1/160K and the end point titre at 1/5M.
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